Table 1.

Quantification of Slouch cluster loss and gain

Cluster I present Cluster II present
GenotypenNumber of hemisegmentsPercentage of hemisegmentsNumber of hemisegmentsPercentage of hemisegments
Loss of Wg function
   Wild type100100100100100
   wgPE6 (25°)1229700
   wgPE6 (18°)11443**3854
   twiGAL4>UASΔNTcf104858200
   twiGAL4;twiGAL4> UASΔNTcf1031009700
   wg21.2 (18°)130816200
   wgNE2 (25°)77536923
   wgPE4 (25°)710000
   wgNE1 (25°)1100000
Cluster I expanded Cluster II expanded
Number of hemisegmentsPercentage of hemisegmentsNumber of hemisegmentsPercentage of hemisegments
Gain of Wg function
   Wild type1000000
   twiGAL4>UASwgE10300103100
   twiGAL4>UASarms101061*0.90106100
   dAPC2ΔS1108*7110100
   dAPC2d40351*435100
   twiGAL4;twiGAL4>UASwg10000100100
   twiGAL4;twiGAL4>UASarm*10810.90108100
   twiGAL4;Dmef2GAL4>UASwg13500135100
   twiGAL4;Dmef2GAL4>UASarm*11200112100
Cluster I present Cluster II present
Number of hemisegmentsPercentage of hemisegmentsNumber of hemisegmentsPercentage of hemisegments
Temporal shifts of Wg expression
   Wild type100100100100100
   hh2110074**7455
   twiGAL4>UASwg;hhAC/hh13C10310310010299
   wgIL114 25°1000000
   wgIL114 18°100100100100100
   wgIL114 18° 13 hours, 25° 2 hours15415410010166
   wgIL114 18° 12 hours, 25° 3 hours565610000
Cluster I present Cluster II present
Number of hemisegmentsPercentage of hemisegmentsNumber of hemisegmentsPercentage of hemisegments
Rescue of wg mutants with Twist
   wgCX41000000
   wgCX4,twiGAL4>UAStwi58223800
   wgCX4,twiGAL4>UAStwi (29°C)84273200
   wgCX4,twiGAL4,Dmef2GAL4>UAStwi70314400
   twiGAL4;twiGAL4>UAStwi,UAStwi11244112100
  • * , cluster I expanded from two to four cells (see text)

  • ** , cluster I also rescued in hemisegments where cluster II was rescued (see text)