Table 2.

ceh-16 represses eff-1/fusogen-mediated cell fusion in the seam cells

Seam cells
AnimalDescriptionH0H1H2V1V2V3V4V5V6T
1-13 ceh-16(–);eff-1(–)
14ceh-16 mosaic; eff-1(–)+++++++
15++++++++
16+
17++++++++
18++++++++
19++++++
20+++++++
21++++++
22+++
23ceh-16 (RNAi); eff-1(–); Ex [eff-1p::gfp]*
24
25
26
27
  • –, no CEH-16::GFP in the nucleus or ceh-16(RNAi); +, CEH-16::GFP in the nucleus.

    Genotypes 1-13, double homozygous animals; 14-22, animals that are mosaic for the rescuing ceh-16::gfp construct, showing suppression at single cell resolution (see also Fig. 3B); 23-27, animals showing ectopic expression of eff-1p::gfp in the seam cells due to mosaic expression of the eff-1p::gfp extrachromosomal array.

    Grey shading indicates ectopic expression of eff-1p::gfp.