smo-1 and ubc-9 inhibit vulval cell fates and interact with lin-1
| Genotype | RNAi* | %Muv† | n‡ |
|---|---|---|---|
| Wild type | NA | 0 | 306 |
| smo-1 (ok359) § | NA | 9 | 94 |
| lin-1(n1790gf)¶ | NA | 14 | 283 |
| smo-1(ok359); lin-1(n1790gf)** | NA | 82 | 120 |
| smo-1(ok359); mek-2(n2678) †† | NA | 17 | 299 |
| Wild type | Control | 0 | 3233 |
| Wild type | smo-1 | 9 | 617 |
| mek-2(n2678) ‡‡ | Control | 0 | 237 |
| mek-2(n2678) ‡‡ | smo-1 | 20 | 55 |
| mpk-1(n2521) §§ | Control | 0 | 676 |
| mpk-1(n2521) §§ | smo-1 | 5 | 558 |
| smg-1(r861); lin-1(e1275) ¶¶ | Control | 2 | 3143 |
| smg-1(r861); lin-1(e1275) ¶¶ | smo-1 | 78 | 404 |
| lin-1(n1790gf) | Control | 6 | 1399 |
| lin-1(n1790gf) | smo-1 | 51 | 376 |
| Wild type | ubc-9 | 0.4 | 976 |
| smg-1(r861); lin-1(e1275) ¶¶ | ubc-9 | 12 | 457 |
| lin-1(n1790gf) | ubc-9 | 27 | 971 |
NA, not applicable.
↵* L4 hermaphrodites were fed HT115(DE3) E. coli transformed with a control plasmid or a plasmid that expresses double-stranded RNA from the indicated gene. Progeny laid on the first and second day of culture on the RNAi bacteria were scored for the Muv phenotype.
↵† Adult hermaphrodites were scored as multivulval (Muv) if they displayed one or more ventral protrusions displaced from the site of the vulva when examined using a dissecting microscope.
↵‡ n, number of hermaphrodites examined.
↵§ smo-1(ok359) homozygous hermaphrodites were sterile, protruding vulva (Pvl), non-blister (non-Bli), non-egg laying defective (non-Egl) self-progeny of smo-1(ok359)/bli-3(e767) egl-30(n686) hermaphrodites.
↵¶ Complete genotype: smo-1(ok359)/bli-3(e767) egl-30(n686); lin-1(n1790).
↵** These hermaphrodites were sterile, Pvl, non-Bli, non-Egl self-progeny of smo-1 (ok359)/bli-3(e767) egl-30(n686); lin-1(n1790) hermaphrodites.
↵†† These hermaphrodites were sterile, non-GFP positive self-progeny of smo-1 (ok359)mek-2(n2678)/hT2g hermaphrodites.
↵‡‡ mek-2(n2678) homozygous hermaphrodites were sterile, non-GFP-positive self-progeny of mek-2(n2678)/hT2g hermaphrodites.
↵§§ Complete genotype: mpk-1(n2521) unc-79(e1068).
↵¶¶ Complete genotype: smg-1(r861) unc-54(r293); lin-1(e1275).