Table 2.

Microarray data of the genes selected in RT-PCR

Gene nameLocusoMCAv-WTAv-ems1Description
SPLAt4g273302044/2045402.50283.52SPOROCYTELESS/NOZZLE
EMS1At5g07280509/5111062.95146.89EXCESS MICROSPOROCYTES1/EXTRA SPOROGENOUS CELLS
TPD1At4g249722046/2047TAPETUM DETERMINANT 1
AMSAt2g169102048/2049348.889.39ABORTED MICROSPORES
MS5At4g209002050/205175.4623.20Male sterility MS5; pollenless3
MS5 likeAt5g443302052/205390.261.51MS5 family protein
MS1At5g222602054/2055Male sterile protein 1
AtMYB32At4g349902056/2057300.9363.67MYB-like protein myb-related protein Y49
AtMYB4At4g386202058/2059161.19179.94Putative transcription factor (MYB4)
MS2At3g119802060/2061690.7812.25Male sterility protein 2 (MS2)
LPT12At3g515902062/2063446.8540.33Lipid transfer protein
ATSTP2At1g073402064/2065174.9060.65Hexose transporter
ATA1At3g429602066/20672090.3829.80Alcohol dehydrogenase (ATA1)
PAB5At1g717702068/2069823.25106.96Polyadenylate-binding protein 5
At3g284702070/2071758.7522.98Myb transcription factor
At3g132202072/2073794.9714.42ABC transporter
At1g695002074/20751636.4716.85Cytochrome P450
At3g237702076/2077497.5317.17β-1,3-glucanase
At1g012802078/2079430.881.96Cytochrome P450
FLFAt5g101402080/2081251.5420.77MADS box protein FLOWERING LOCUS F (FLF)
At3g605802082/2083245.2722.71Zinc finger protein-like ZPT3-3, Petunia hybrida
ATMYB103At5g561102084/208593.2036.35Regulate the tapetum and trichome development, anther specific gene
A6At4g140802098/20993692.1141.2Glycosyl hydrolase family 17 protein / anther-specific protein (A6)
A9At5g072302100/21015099.7981.5Protease inhibitor/seed storage/lipid transfer protein, tapetum-specific protein A9
MYB33At5g061002102/2103238.76176.9Myb family transcription factor (MYB33)
MYB65At3g114402104/2105335.48203.4Myb family transcription factor (MYB65)
At1G061702127/2128938.3528.1Basic helix-loop-helix (bHLH) family protein
At2G312102129/2130176.0820.6Basic helix-loop-helix (bHLH) family protein
SDSAt1g14750271/27280.4827.18SOLO DANCER, a putative cyclin
RCK/AtMER3At3g277301963/196481.6837.16ROCK-N-ROLLERS/AtMER3, a ATP-dependent DNA helicase
UBQ1At3g52590U1/U24509.454007.97UBIQUITIN EXTENSION PROTEIN 1 (UBQ1)
  • The genes were selected as described.

    The microarray intensity value of the gene for the ems1/exs anther is at most half of that from the wild-type anther. Genes known to be important for early anther development are regulatory genes SPL/NZZ, TPD1, AtMYB33 and AtMYB65. Constitutive controls are AtMYB4 and the UBQ1.