Table 1.

Expression of marker genes specific for NB subsets in wt, vnd loss-of-function (vnd-) and gain-of-function (sca-vnd) embryos at stage 11

Wild type* vnd- sca-vnd
divd%i%v%d%i%v%
Msh Dv2 23
Dv3 82
Dv4 35
Dv5 15
Dd2-13Dd2-13100 Dd1 91 Dv6 80 Dd2-13 0
Dv7 9
Dv8 30
Td4-8Td4-8100 Td2 75 Tv1,4 5 Td4-8 0
Tv2 25
Tv3 15
Tv5 30
ind (Ppd6,9,13 Pcd7,13)(Ppd6,9,13 Pcd7,13)All 100 2-3 PCNBs
Dd1 Dd1 0 Dd1 92
Td1-3 Td1,2 0 Td1-3 0
Td3 85
EyPad7Pcv6Pad7 Pcv6 55 Pad7 Pcv6100
Pcd2,14Pcv7Pcd2,14Pcv795 Pcd2,14 Pcv7100
Ppd12,18,Pcv9Ppd12,18,Pcv9100 Pcd12,18,20 Pcv9100
20Ppv220 Ppv2 35 Ppv2 55
Dd4Td1Dv6Dd4 Td1 0 Dv6 0 Dd4 Td1 0 Dv6100
Td2Dv7All 100 Td2 40 Dv7 10 All <5 Td2 0 Dv7 20
DacPcd1,2,4,Pav1Pcd1,2,4,Pav1100 Pcd1,2,4, Pav1 40
6,8,9,14Pcv76,8,9,14Pcv7,9100 6,8,9,14 <20 Pcv7100
Ppd1,9,11Pcv9Ppd1,9,11 2-4 PCNBs Ppd1,9,11 <20 Pcv9100
Tv2All 100 Tv2 25 Pcd2100Tv295
EmsPpd2,5,8Pcv5Ppd2,5,8 Pcv5 15 Ppd2 30 Pcv5 88
Ppd5 0
Ppd8 20
Dd3,6,8Dv3Dd3,6,8 Dd1 80 Dv3 85 Dd3 12 Dv3100
Dv2 20 Dd6 87
Td6 Dv6 70 Dd8 92
Td6All 100 Tv2 25 Td6 0
LbePcv8 Pcv8 0 1-6 PCNBs Pcv8100
Dd7Ppv3Dd7 Ppv3 0 Dd7 40 Ppv3100
Td4Td4All 100 Td4 0
EnPpd5,8Dv8Ppd5,8 Dv8 30 Ppd5 75
Dd5Tv4,5Dd5 Tv4 5 Ppd8 30
Td5Td3Td5 Td3 90 Tv5 30 Dd5 25 Dv8 75
All 100Td5100Td3100Tv4,5100
Gsb-dPpd3,10Ppd3,10n.d.n.d.n.d.
Dd5,7Dd5,7 Td2 75
Td4Td2,3Tv3Td4All 100 Td3 75 Tv3 11
WgPcd3,6,7,--Pcd3,6,7,-- Pcd3,6,7, 10 --
10-13,10-13, 10-13, 10
16-1916-19 Pcd16-19 90
Ppd1,3,4,Ppd1,3,4, Ppd1,3,4, 10
6,7,96,7,9 6,7,9 10
Dd1,7,8Dd1,7,8 Dd1,7,8 45
Td4Td4All 100 Td4 35
Svp-lacZPad1,7,8,Pcv1,3,Pad1,7,8, Pcv1 85 n.d.n.d.n.d.
156,7,915 Pcv3 18
Pcd2,4,6,Pcd2,4,6, Pcv6 55
9,18,219,18,21 Pcv7 85
Ppd3,9,15,Ppd3,9,15, Ppv2 25
16,18,20Ppv216,18,20Pcv9100
Ppv2 18
Dd1 Dd1 90 Dv2 27
Dd3,6,7,8,Dv2-8Dd3,6,7,8, Dv3,6 82
1313 Dv4,8 35
Td6,8Td6,8 Dv5 15
Tv2All 100 Dv7 9
Tv2 25
AtoPpd19-Ppd19- Dv1 25 n.d.n.d.n.d.
Dd9,11,Dv1,3Dd9,11, Dv3 5
12,1312,13All 100
  • Percentages indicate presence of marker in vnd— or sca-vnd (n=16-30 in each case).

    Bold indicates marker expression less than 90%.

    Bold italics indicate ectopic gene expression.

    PCNBs, protocerebral NBs;—, marker gene is not expressed; n.d., not determined; d, dorsal; i, intermediate; v, ventral.

  • * In wild type, marker genes are expressed in respective NBs always to 100%.

  • Position of ocular ind domain is exceptional to other ind domains; it is unclear whether descending Ppd6,9,13, Pcd7,13 represent intermediate NBs.