Table 2.

Regulation of dauer formation and germline proliferation

Genotype% Dauer arrest (n)Number of germ cell nuclei in dauer*(n)
(A) N20 (124)39.92±4.58 (25)§
(B) aak-2(ok524)0 (148)60.36±9.30†A (25)§
(C) daf-2(e1370)100 (293)35.44±3.85 (25)
(D) daf-2(e1370); aak-2(rr48)100 (156)96.16±18.06†C (25)
(E) daf-2(e1370); aak-2(ok524)100 (290)69.96±9.08†C (25)
(F) daf-2(e1370); aak-1(RNAi)100 (95)47.77±9.26†C (30)
(G) daf-2(e1370); daf-18(e1375)99.48 (763)98.17±13.61†C (30)
(H) daf-2(e1370); akt-1(mg144)gf97.7 (607)50.82±6.57†C (28)
(I) daf-2(e1370); akt-1(mg144)gf; aak-2(rr48)77.4 (1387)126.60±18.95†D (15)
(J) daf-2(e1370); aak-2(rr48); aak-1(RNAi)ND165.00±36.13†D (28)
(K) daf-2(e1370); akt-1(mg144)gf; aak-2(ok524)67.7 (653)85.33±12.40†E (15)
(L) daf-2(e1370); aak-2(ok524); aak-1(RNAi)ND152.64±32.51†E (25)
(M) daf-2(e1370); daf-18(e1375); aak-2(ok524); aak-1(RNAi)85.13 (316)189.53±23.71†L (15)
(N) daf-7(e1372)100 (182)33.19±4.07 (26)
(O) daf-7(e1372); aak-2(rr48)100 (201)80.12±6.44†N (25)
(P) daf-7(e1372); aak-2(ok524)100 (153)65.24±14.85†N (25)
(Q) daf-7(e1372); aak-1(RNAi)100 (311)44.60±10.68†N (25)
(R) daf-7(e1372); daf-18(e1375)99.8 (577)87.24±14.41†N (25)
(S) daf-7(e1372); daf-18(ok480)92.8 (376)177.48±21.48†N (25)
(T) daf-7(e1372); akt-1(mg144)gf100 (423)32.15±2.60‡N (26)
(U) daf-16(mgDf50); daf-7(e1372)98.7 (234)39.68±4.89†N (25)
(V) daf-16(mgDf50); daf-7(e1372); aak-2(rr48)32.0 (222)95.24±10.65†U (25)
(W) daf-16(mgDf50); daf-7(e1372); aak-2(ok524)50.5 (497)82.93±10.14†U (15)
  • n, sample size.

    ND, not determined.

  • * , Mean values±s.d.

  • , Statistical significance using the one-tailed t-test with unequal variance (P≤0.0005), versus row `X'.

  • , As above but, P=0.14.

  • § , Dauer formation (>90% daf-c) was induced using dauer pheromone preparation.