Table 1.

PH3 and TUNEL analysis in the rostral midline and rostroventral telencephalon

Marker assayedGenotypeNumber of embryosBox 1Box 2
PH3Wild type31.4±1.226±5.7
PH3Null/Neo21.5±0.7 [0.37]12±4.6 [0.016]
PH3TelKO21.7±0.6 [0.39]13±3.7 [0.003]
TUNELWild type35±1.22.5±1.3
TUNELNull/Neo21.6±0.9 [0.005]38±18 [0.005]
TUNELTelKO25.5±1.2 [0.37]38±20 [0.001]
  • The data show the average number±s.d. of cellular nuclei labeled with the M-phase marker (phosphohistone 3; PH3) and the average number of TUNEL+ profiles (apoptosis marker) in the rostral midline (Box 1) and in the rostroventral telencephalon (Box 2) (see PH3 and TUNEL data in Figs 3, 4, 5, 6). We do not know whether or not an individual cell can have more than one TUNEL+ signal. See Materials and methods for a description of how the boxes were drawn. Averages were determined by counting the total number of labeled cells in Box1 or Box2 (which was drawn to both the left and the right of the midline) in three or four sections of two or three embryos and dividing by the number of boxes counted. The standard deviation (in parentheses) reflects the variance in the average number of positive cells/embryo. Results of T-test analyses are shown in square parentheses for each mutant in comparison with wild type. Values below 0.05 are statistically significant.