Table 1.

Summary of combinatorial cyp26 knock-down experiments and their outcomes

Summary of phenotype
TreatmentCyp26a1+Cyp26a1−/−
5 ng control MONoneVery mild (expanded r4)
2.5 ng cyp25b1 MO1 + 2.5 ng control MONoneVery mild (expanded r4)
2.5 ng cyp25c1 MO1 + 2.5 ng control MONoneMedium (up to cerebellum)
2.5 ng cyp25b1 MO1 + 2.5 ng cyp26c1 MONoneSevere (up to cerebellum)
5 nM RANoneSevere (throughout brain)
10 μM DEAB + 5 nM RANoneSevere (throughout brain)
Morpholino sequences:
Cyp26b1 MO1 (ATG)5′-CTCGAAGAGCATGGCTGTGAACGTC-3′
Cyp26b1 MO2 (exon-2-intron-2 splice)5′-ATTGACCTTACCTTCCTCCTTTTGC-3′
Cyp26c1 MO1 (exon-3-intron-3 splice)5′-AAACTCGGTTATCCTCACCTTGCGC-3′
Cyp26c1 MO2 (intron-1-exon-2 splice)5′ GGAACCCTGTCACAACATAACAGAG-3′
Control (dead-end MO)5′-GCTGGGCATCCATGTCTCCGACCAT-3′