Table 1.

Levels of Dkk1 and Wnt3 expression in embryos of (A) Wnt3 and (B) Dkk1;Wnt3 crosses as determined by quantitative RT-PCR

Expression level
Genotype of embryo Dkk1 (n) Wnt3 (n)
A. Wnt3+/− × Wnt3+/−
Dkk1 +/+ Wnt3 +/+ 99032±10365 (7) [100%] 6039±1294 (7) [100%]
Dkk1 +/+ Wnt3 +/− 101383±12083 (6) [102%] 5111±1995 (6) [84%]
Dkk1 +/+ Wnt3 −/− 5552±1871** (8) [5.6%] 118±60** (8) [1.9%]
B. Dkk1+/−;Wnt3+/−× Dkk1+/−;Wnt3+/−
Dkk1 +/+ Wnt3 +/+ 2265±275 (8) [100%] 306±86 (8) [100%]
Dkk1 +/+ Wnt3 +/− 2038±449 (9) [90%] 175±49 (9) [57%]
Dkk1 +/− Wnt3 +/+ 1617±317 (5) [71%] 201±110 (5) [66%]
Dkk1 +/− Wnt3 +/− 1027±167* (7) [45%] 149±34 (7) [49%]
  • *P<0.01, **P<0.01 by Student's t-test compared with Dkk1+/+;Wnt3+/+ level. The difference in the absolute values of the expression level between datasets A and B is due to the use of RT-PCR machines with different settings. However, each set is internally consistent as the samples were analyzed under similar conditions using the same instrument.

  • Expression level: mean±s.e. arbitrary units normalized relative to GAPDH [%].