Table 2.

The number of E7.0-E10.5 embryos of different genotypes derived from Dkk1+/−;Wnt3+/− intercross

Number of embryos of each genotype*(number displaying abnormalities)
Dkk1+/+Dkk1+/+Dkk1+/−Dkk1+/−Dkk1+/+Dkk1+/−Dkk1−/−Dkk1−/−Dkk1−/−
Age (E)Wnt3+/+Wnt3+/−Wnt3+/+Wnt3+/−Wnt3−/−Wnt3−/−Wnt3+/+Wnt3+/−Wnt3−/−Total
7.0-8.01 (0)3 (0)1 (1)8 (8)5 (5)4 (4)1 (1)4 (4)3 (3)30
8.0-9.04 (0)12 (1)4 (1)8 (6)7 (7)6 (6)2 (2)3 (3)2 (2)48
9.512 (0)24 (1)17 (1)45 (31)12 (12)12 (11)4 (4)10 (10)3 (3)139
Total number17 (0)39 (2)22 (3)61 (45)24 (24)22 (21)7 (7)17 (17)8 (8)217
% Abnormal05147410095100100100
10.511 (0)19 (1)21 (2)26 (3)007 (7)22 (16)0106
% Abnormal051012N/AN/A10073N/A
  • Embryos of three age groups (E7.0-8.0, E8.0-9.0 and E9.0-10.0) were collected. The number of embryos of each genotype is shown, followed by the number (in parentheses) that displayed abnormal phenotype; the percentage of abnormal embryos in each genotype class is shown in the last row of each intercross.

    N/A, not applicable as no embryos of this genotype were found.

  • * ϰ2 analysis was performed on the frequency of each genotype across all age groups, but genotypes were not present at the expected Mendelian frequency (ϰ2=30.5, significant difference at P<0.05) due to the loss of Wnt3-null embryos, which were arrested at gastrulation.