Table 1.

Apoptosis and mitosis in the cultured PGCs

A Apoptosis
Treatment % Apoptosis χ2 test
bFGF 4OHT Wild type AKT-MER− 4OHT Wild type
8.1% (25/309)8.2% (30/365)>0.05
+6.3% (22/348)2.0% (8/395)<0.005<0.005
+2.2% (8/369)3.0% (10/337)>0.05
+ + 2.2% (8/362) 0.33% (1/302)<0.025<0.05
B Mitosis
Treatment % Mitosis χ2 test
bFGF 4OHT Wild type AKT-MER− 4OHT Wild type
0% (0/309)0% (0/365)n.a.
+0% (0/348)1.0% (4/395)>0.05>0.05
+0.84% (3/369)0.93% (3/337)>0.05
++0.85% (3/362)4.2% (12/302)<0.01<0.01
  • PGCs of E11.5 embryos were cultured for 2 days under the indicated conditions. The apoptotic cells were labeled for 1 hour with SR-VAD-FMK before fixation. The PGCs were visualized with SSEA-1 staining, and mitotic cells were identified with DAPI staining.

    n.a., not applicable.