Table 3.

Effects of PI3K inhibition and AKT activation on EG cell derivation

TreatmentSeeded PGC (embryo/well)EG cell-positive wells
4OHTLY294002Wild typeAKT-MER
0.027/126/12
+0.020/120/12
+0.026/1212/12
++0.020/1212/12
+0.005nd7/12
++0.005nd9/12
+0.0025nd5/12
++0.0025nd4/12
  • Cell suspensions from the genital ridges of E11.5 embryos were seeded onto SI/SI4-m220 feeder cells at a density of 0.0025-0.02 embryos per well. The cells were cultured with bFGF and LIF in the presence of 1 mM 4OHT and/or 10 μM LY294002.

    nd, not done.