Table 4.

Production of chimeric mice

EG cell lineCondition for EG cell establishmentNo. of embryos transferredDead non-chimeraDead chimeraLive non-chimeraLive chimeraChimeras/total
M1-3 −bFGF, SCF, LIF 18 0 2 2 1 * 3/5
18 1 0 8 0 0/9
M1-5 −bFGF, SCF, LIF 22 0 1 2 2 3/5
26 1 2 2 0 2/5
M1-7 −bFGF, SCF, LIF 18 0 3 1 1 4/5
28 0 0 0 0 0/0
L-2 −bFGF, −SCF, LIF 21 1 0 6 0 0/7
18 0 0 2 0 0/2
  • * Chimeric mouse died soon after birth.

  • One chimeric mouse survived to adulthood, whereas one chimera died soon after birth.

  • Chimeric mouse survived to adulthood.