Table 5.

Effects of AKT activation on EG cell derivation from E13.5-E16.5 germ cells

EG cell-positive wellsχ2 test
Embryonic daySexForskolinSeeded PGC (embryos/well)4OHT (nM)Wild typeAKT-MERWild type0 nM
E13.5M0.0204/125/12nsd
0.023005/1212/12<0.005<0.005
F0.0201/121/12nsd
0.023002/122/12nsdnsd
M+0.0205/126/12nsd
+0.023006/1212/12<0.005<0.005
F+0.0204/123/12nsd
+0.023003/128/12<0.05<0.05
E14.5M+0.100/120/12nsd
+0.11000/125/12<0.025<0.025
+0.13000/124/12<0.05<0.05
+0.110000/123/12nsdnsd
F+0.100/50/5nsd
+0.13000/50/5nsdnsd
E15.5M+0.100/100/10nsd
+0.11000/100/10nsdnsd
F+0.100/50/10nsd
+0.11000/50/30nsdnsd
+0.13000/70/10nsdnsd
E16.5M+0.100/120/12nsd
+0.11000/120/12nsdnsd
F+0.100/120/12nsd
+0.11000/120/12nsdnsd
  • Cell suspensions from the gonads of E13.5 to E16.5 embryos were seeded onto SI/SI4−m220 feeder cells and cultured with bFGF, LIF and forskolin in the presence of various concentrations of 4OHT. The cells of E13.5 embryos were also cultured without forskolin.

    M, male; F, female; nsd, not statistically different.