Table 2.

Primers for RealTime qPCR (Dlx and other SHFM mRNAs in mouse limbs)

GeneOrientationSequence (5′ to 3′)
GAPDH STGTCAGCAATGCATCCTGCA
GAPDH ASTGTATGCAGGGATGATGTTC
Dlx1 STCCGAGAAGAGTACGGTGGT
Dlx1 ASACTTGGAGCGTTTGTTCTGG
Dlx2 STGGGCTCCTACCAGTACCAC
Dlx2 ASTGGTTTCCGGACTTTCTTTG
Dlx5 SCTGGCCGCTTTACAGAGAAG
Dlx5 ASCTGGTGACTGTGGCGAGTTA
Dlx6 SAGAGCGCTTATTCTGAAACCAT
Dlx6 ASCTCAATACCTGGCCCTTCC
Dlx6-antisense SGGGTGACCACTCCTCTGTGT
Dlx6-antisense ASCTAAGGAAGCAGCCAGTTCG
Evf2 SGCTTCAAATTCCTACTGTGA
Evf2 ASTTTCCCCCTTCCTACTGTGA
Dactylyn SGTTGTGGGCACTTCTCACCT
Dactylyn ASGCAGTGTAGAGGGGGACTCA
ΔNp63SATGTTGTACCTGGAAAACAATG
ΔNp63ASGATGGAGAGAGGGCATCAAA
TAp63 SAGACAAGCGAGTTCCTCAGC
TAp63 ASTGCGGATACAATCCATGCTA
Perp SCCCAGATGCTTGTTTTCCTG
Perp ASACCAGGGAGATGATCTGGAAT
  • AS, antisense; S, sense.