Table 4.

Expression of Dlx genes in limbs of p63 mutant embryos

A
Target mRNA Dlx1 Dlx2 Dlx5 Dlx6 Dlx6as Dactylyn Perp
FL *
+/+1111111
p63 −/− 0.60.50.50.30.51.20.2
p63EEC/EEC 0.5 0.4 0.3 0.3 2 1.5 0.55
HL *
+/+1111111
p63 −/− 0.350.250.250.311.30.3
p63EEC/EEC 0.25 0.25 0.2 0.2 3 1.3 0.4
B
Target mRNA Dlx1 Dlx2 Dlx5 Dlx6 Perp
FL
+/+11111
p63 +/− 1.21.61.51.31.0
p63+/EEC 1.2 1.3 2 1.1 1.0
HL
+/+11111
p63 +/− 0.60.60.50.50.5
p63+/EEC 0.90.90.60.550.7
  • * FL, forelimb; HL, hindlimb. The genotypes of p63 (Brdm2 strain) and p63EEC (R279H) mutant limbs are indicated.

  • Values are normalized to the wild-type (+/+) sample, set=1. An external calibration sample was used, GADPH mRNA was determined as a positive control reference. Most determinations were repeated three times.