Table 1.

Genes implicated in epidermal differentiation that are downregulated in the epidermis following 24-hour culture with EGF or KGF ligand

GeneControlKGFHBEGF
sonic hedgehog0−9−9
dickkopf homolog 4 (Xenopus laevis)0−9−13
GLI-Kruppel family member GLI10−8−10
periostin, osteoblast specific factor0−50
patched homolog 20−5−12
bone morphogenetic protein 20−3−12
cut-like 10−3−3
lymphoid enhancer binding factor 10−2−2
  • Embryonic skin was cultured with BSA (control) (250 ng/ml), HBEGF (250 ng/ml) or KGF (250 ng/ml) for 24 hours and genomic transcript expression was profiled by microarray. A number of genes linked to major pathways (Wnt, sonic hedgehog, BMP) involved in HF morphogenesis are downregulated following HBEGF or KGF treatment. 0, baseline levels or no change; numbers, fold of baseline.