Table 1.

MS-dependent tissues produced in wild-type and mutant embryos

GenotypePharynx cells (pha-4::GFP)Pharynx muscles (ceh-22::GFP)Muscle cells (hlh-1::GFP)Coelomocytes (cup-4::GFP)
Wild type50.0±0.9 (21)12.8±0.1 (37)44.7±1.1 (20)3.7±0.2 (105)
skn-1 (RNAi) 4.8±0.4 (20)0.0±0.0 (165)nd0.15±0.04 (124)
pal-1 (RNAi) 49.5±0.8 (10)11.7±0.3 (12)21.6±0.9 (13)3.7±0.1 (103)
pop-1 (RNAi) ndndnd0.0±0.0 (50)
glp-1 (RNAi) 23.1±0.6 (15)5.7±0.2 (38)ndnd
tbx-35(tm1789) 15°C40.6±1.2 (17)5.9±0.3 (24)37.3±1.6 (10)3.8±0.2 (28)
tbx-35(tm1789) 23°C35.7±0.8 (16)**5.2±0.2 (46)*34.8±2.4 (10)3.3±0.4 (20)
tbx-35(tm1789); glp-1 (RNAi) 15°C6.6±0.5 (23)2.0±0.4 (26)ndnd
tbx-35(tm1789); glp-1 (RNAi) 23°C1.1±0.3 (32)**1.0±0.2 (39)*ndnd
tbx-35(tm1789); pal-1 (RNAi) 15°C38.8±0.7 (15)5.1±0.3 (14)8.4±1.0 (17)2.2±0.2 (47)
tbx-35(tm1789); pal-1 (RNAi) 23°C35.6±1.0 (14)*4.7±0.3 (17)5.7±0.5 (40)*0.6±0.1 (49)**
ceh-51 (tm2123) 47.8±0.9 (17)9.2±0.2 (10)42.4±1.4 (10)2.1±0.1 (53)
ceh-51 (tm2123); pal-1 (RNAi) ndnd19.3±0.5 (11)2.5±0.1 (84)
med-1 (ok804); med-2(cx9744) 31.3±0.6 (26)4.1±0.2 (32)31.0±2.7 (10)0.07±0.03 (34)
ceh-51 (tm2123); tbx-35(tm1789) 30.2±0.5 (44)4.4±0.2 (18)30.1±1.0 (14)0.19±0.04 (124)*
med-1 (ok804); med-2(cx9744); glp-1 (RNAi) 1.4±0.4 (14)0.3±0.1 (31)ndnd
ceh-51 (tm2123); tbx-35(tm1789); glp-1 (RNAi) 1.9±0.5 (26)0.5±0.1 (52)ndnd
med-1 (ok804); med-2(cx9744); pal-1 (RNAi) ndnd3.8±0.5 (13)nd
ceh-51 (tm2123); tbx-35(tm1789); pal-1 (RNAi) ndnd3.9±0.4 (15)nd
  • Strains were grown at 20-23°C unless otherwise indicated. Data are shown as the mean ± s.e.m. *0.01<P<0.05, **P<0.01, Student's t-test, by comparison with the experiment immediately above. nd, not done.

  • Only pharynx expression of pha-4::GFP, anterior to the gut (when present), was scored.

  • The anatomy of the pharynx was considered in assigning expression to particular muscle cells.