Table 2.

The brom bones dorsal axial defects are cold sensitive

Incubation temperature (°C)Embryos scored at 1 dpfVentralized embryos
GenotypeIIIIIINormal embryos
Wild type*28.554000100%
2091000100%
brb128.5408%10%082%
201817%39%11%33%
brb228.53110%13%077%
203418%17%6%59%
brb328.53152%19%029%
2040100%000
  • I: Severely ventralized embryos were radially ventralized or headless, as shown in Fig. 1H,I.

    II: Intermediately ventralized embryos displayed a partially reduced head and lacked a notochord, as shown in Fig. 1J.

    III: Mildly ventralized embryos exhibited a reduced head and eyes, as shown in Fig. 1K.

    brb1, brb2, brb3 were three homozygous brom bones females used in the experiment.

  • * Embryos from two wild-type females were scored.

  • Mutant embryos that died before 1 dpf due to egg activation defects are not included in the total number of embryos scored.