Self-renewal
- Defined conditions for propagation and manipulation of mouse embryonic stem cells
Summary: Detailed protocols for the propagation, manipulation and differentiation of mouse embryonic stem cells, with tips for minimising variability and improving the efficiency, reproducibility and reliability of embryonic stem cell culture.
- Neural stem cells: origin, heterogeneity and regulation in the adult mammalian brain
Summary: This Review discusses the identification, regulation and heterogeneity of neural stem cells and examines recent insights into their transcriptomic properties and mechanism of persistence into adulthood.
- Two distinct mechanisms silence chinmo in Drosophila neuroblasts and neuroepithelial cells to limit their self-renewal
Summary: The transcription factor chinmo acts as a master gene of neural stem cell self-renewal in the different regions of the developing Drosophila brain, where it is controlled by distinct regulatory strategies.
- Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors
Summary: The transcription factor Ascl2 is identified as a novel inhibitor of myogenic regulatory factors in mice, acting to inhibit myogenic differentiation and facilitate the generation of postnatal satellite cells.
- DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation
Highlighted article: DNA damage disrupts stem cell self-renewal and differentiation in a CHK2 kinase-dependent manner, which may be due to reduced BMP signaling and cell adhesion in the Drosophila ovary.
- A role for post-transcriptional control of endoplasmic reticulum dynamics and function in C. elegans germline stem cell maintenance
Summary: PUF-8 promotes germline stem cell proliferation by promoting the expression of FARL-11, an ER protein that facilitates the membrane localization of GLP-1 /Notch.
- Protein synthesis and degradation are essential to regulate germline stem cell homeostasis in Drosophila testes
Summary: A large-scale RNAi screen in Drosophila testes reveals that many genes involved in protein synthesis and degradation are required for the homeostasis of germline stem cells and their niches.
- The LGN protein promotes planar proliferative divisions in the neocortex but apicobasal asymmetric terminal divisions in the retina
Highlighted article: LGN inactivation in mice disrupts spindle orientation in both the retina and neocortex but leads to very different outcomes with regards to cell fate in each context.
- Intrinsic and extrinsic mechanisms regulating satellite cell function
Summary: This Review discusses how satellite stem cell behaviour is regulated during regeneration and degeneration by a complex balance between extrinsic cues and intrinsic regulatory mechanisms.
- p53 enables metabolic fitness and self-renewal of nephron progenitor cells
Highlighted article: Although classically thought to restrain proliferation, p53 promotes self-renewal in nephron progenitors of the mouse kidney and also regulates their metabolic profile.